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Plant regeneration and Agrobacterium-mediated transformation of Achyranthes bidentata using cotton EREBP gene BABT
Duan,Hong Ying; Ding,Xiao Sheng; Song,Jian Ying; He,Yun Long; Zhou,Yan Qing.
The aim of this work was to study the plant regeneration and Agrobacterium-mediated transformation of Achyranthes bidentata using cotton EREBP gene. Results showed that the callus induction rate of stems from A. bidentata was the highest (100%) and bud was in approximately 70% of calli from stems. However bud differentiation rate of the callus from leaves and petioles was very low. Compared with ceftriaxone, 200mg/L cefotaxime could completely control Agrobacterium tumefaciens and had relatively less toxic action on the stems of A. bidentata. In addition, the induction rate of callus resistant to hygromycin was the highest when infected for 3 min and co-cultivated for 3 d. Six positive transgenic plants transformed with pCAMBIA1304-GhEREB2 expression...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Achyranthes bidentata; EREBP gene; Agrobacterium tumefaciens; Genetic transformation.
Ano: 2013 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132013000300001
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